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AAT Bioquest

FAQ

Posted on March 21, 2024
What are the advantages of LDH release assay?
Apoptosis and NecrosisCell CytotoxicityCellular ProcessesDehydrogenaseEnzymesLDHlactate dehydrogenase


Posted on March 21, 2024
What are the drawbacks of LDH release assay?
Apoptosis and NecrosisCell CytotoxicityCellular ProcessesEnzymesLDHlactate dehydrogenase


Posted on March 12, 2024
What are the commonly used methods for measuring cytotoxicity?
Chemical ReagentsATPCell CytotoxicityCellular ProcessesPhysiological Probes


Posted on March 12, 2024
What are the applications of cytotoxicity assessment?
Chemical ReagentsCell CytotoxicityCellular ProcessesPhysiological Probes


Posted on July 17, 2023
What are the applications of trypan blue?
Cell CytotoxicityCell Viability AssaysCellular Processes


Posted on June 21, 2023
What is the principle of LDH assay?
Cell CytotoxicityDehydrogenaseEnzymesLDHcell damagelactate dehydrogenase


Posted on June 21, 2023
What is the principle of CVS assay?
Apoptosis and NecrosisCell CytotoxicityCell Viability AssaysCellular ProcessesDNAcell death


Posted on June 21, 2023
What is the principle of fluorometric cell viability assays?
Chemical ReagentsApoptosis and NecrosisCell CytotoxicityCell Viability AssaysCellular ProcessesPhysiological Probes


Posted on June 21, 2023
What are the limitations of colorimetric assays of cell viability and cytotoxicity?
Apoptosis and NecrosisCell CytotoxicityCell Viability AssaysCellular ProcessesLive or DeadPhysiological Probes


Posted on June 21, 2023
What are the limitations of luminometric cell viability assays?
Chemical ReagentsCell CytotoxicityCell Proliferation AssaysCell SignalingCell Viability AssaysCellular ProcessesPhysiological Probesluminescense


Posted on June 21, 2023
What are the advantages of fluorometric assays of cell viability and cytotoxicity?
Chemical ReagentsApoptosis and NecrosisCell CytotoxicityCell SignalingCellular ProcessesLive or Dead


Posted on June 21, 2023
What are the pros and cons of CVS assay (crystal violet assay)?
Chemical ReagentsCell CytotoxicityCell SignalingCell Viability AssaysCellular Processes


Posted on June 21, 2023
What is the principle of MTT assays?
Chemical ReagentsCell CytotoxicityCell Proliferation AssaysCell SignalingCell Viability AssaysCellular Processes


Posted on June 21, 2023
What are the advantages of XTT assays?
Chemical ReagentsCell CytotoxicityCell Proliferation AssaysCell SignalingCell Viability Assays


Posted on June 21, 2023
What is the principle of WST-8 assays?
Cell CytotoxicityCell Proliferation AssaysCell SignalingCell Viability AssaysCellular ProcessesWST-8


Posted on May 3, 2023
What are the functions of cell signaling?
Cell Cycle AnalysisCell Cycle and ProliferationCell CytotoxicityCell MetabolismCell SignalingCell ViabilityCellular ProcessesG-Protein-Coupled Receptors (GPCR)Membrane Potential and Channels


Posted on May 3, 2023
How do cells communicate?
Apoptosis and NecrosisCell Cycle AnalysisCell Cycle and ProliferationCell CytotoxicityCell MetabolismCell SignalingCell ViabilityCellular ProcessesG-Protein-Coupled Receptors (GPCR)Membrane Potential and Channels


Posted on May 3, 2023
What are the common outcomes of cell signaling?
Apoptosis and NecrosisCell Cycle AnalysisCell Cycle and ProliferationCell CytotoxicityCell MetabolismCell SignalingCellular ProcessesEnzymes


Posted on November 16, 2022
Is DAPI soluble in PBS?
Antibody and Protein LabelingCell Cycle AssaysCell CytotoxicityCell Proliferation AssaysCell Viability AssaysCellular Processes


Posted on November 16, 2022
Can DAPI stain dead cells?
Antibody and Protein LabelingBioconjugationCell Cycle AssaysCell CytotoxicityCell Proliferation AssaysCell Structures and OrganellesCellular Processes


Posted on November 16, 2022
What does MTT measure?
BioconjugationCell CytotoxicityCell Proliferation AssaysCell Viability AssaysCellular Processes


Posted on August 23, 2022
How do I measure cell cytotoxicity?
BioconjugationCell CytotoxicityCell Proliferation AssaysCell Viability AssaysEnzymes


Posted on August 11, 2022
What does a CD45 marker stain?
Cell CytotoxicityCellular ProcessesPhysiological ProbesCluster of Differentiation (CD Antibodies)


Posted on August 9, 2022
What are the differences between ADP and ATP?
Cell CytotoxicityPhysiological ProbesApoptosis and Necrosis


Posted on August 5, 2022
What are the common types of epithelial tissues?
Physiological ProbesCellular ProcessesCell Cytotoxicity


Posted on August 4, 2022
How does flow cytometry work?
Flow Cytometry ReagentsCell CytotoxicityPhysiological Probes


Posted on August 3, 2022
What light source is used in most flow cytometers?
Cell CytotoxicityFlow Cytometry ReagentsCellular ProcessesCell MetabolismPhysiological Probes


Posted on August 3, 2022
What is the purpose of a fluorescent marker?
Physiological ProbesCell CytotoxicityCellular ProcessesCell Metabolism


Posted on August 1, 2022
Do all cells have a cell wall?
NucleusCell Structures and OrganellesBioconjugationCell Cytotoxicity


Posted on July 27, 2022
What's the difference between endocytosis and phagocytosis?
Physiological ProbesCell CytotoxicityMembrane Potential and ChannelsCellular Processes


Posted on July 27, 2022
What is phagocytosis?
Cell CytotoxicityCellular ProcessesMembrane Potential and ChannelsPhysiological Probes


Posted on July 22, 2022
Where does splicing occur?
DNA and RNA QuantitationCellular ProcessesCell CytotoxicityNucleus


Posted on July 19, 2022
What are the products of fatty acid oxidation?
Membrane Potential and ChannelsPhysiological ProbesCell CytotoxicityCellular Processes


Posted on July 14, 2022
What is the difference between MTT and resazurin?
Cell CytotoxicityReactive Oxygen Species (ROS)Cellular Processes


Posted on July 12, 2022
How does propidium iodide measure the cell cycle?
DNA and RNA QuantitationPhysiological ProbesCellular ProcessesCell CytotoxicityCell Cycle Assays


Posted on July 12, 2022
What is the alternative to propidium iodide for dead cell staining?
Cell CytotoxicityCellular ProcessesBioconjugationFlow Cytometry ReagentsCell Cycle AssaysDNA and RNA Quantitation


Posted on July 8, 2022
Is CD45 a phosphatase?
Physiological ProbesAntibody LabelingAntibodies and ProteomicsCell Cytotoxicity


Posted on July 1, 2022
Where does fatty acid oxidation occur?
Cell CytotoxicityCellular ProcessesPhysiological Probes


Posted on July 1, 2022
What is the most common coenzyme?
EnzymesCell CytotoxicityCell MetabolismCellular ProcessesPhysiological Probes


Posted on June 6, 2022
Why are bacterial cell walls important?
Physiological ProbesCellular ProcessesCell Structures and OrganellesDNA and RNA QuantitationCell Cytotoxicity


Posted on May 24, 2022
What kinds of assays use 7-AAD?
Cell CytotoxicityCell Cycle AssaysCell Cytotoxicity


Posted on May 24, 2022
How does 7-AAD bind to DNA?
Flow Cytometry ReagentsCell ViabilityCell Cycle AssaysCell Cytotoxicity


Posted on May 24, 2022
Is 7-AAD cell-permeable?
Cell Cycle AssaysCell CytotoxicityCellular ProcessesCell ViabilityFlow Cytometry Reagents


Posted on May 24, 2022
Can 7-AAD be fixed?
Cell Cycle AssaysCell CytotoxicityCellular ProcessesCell ViabilityFlow Cytometry Reagents


Posted on May 16, 2022
What are the structural features of prokaryotic cells?
Cellular ProcessesCell CytotoxicityCell Structures and Organelles


Posted on May 13, 2022
What are the components of prokaryotic cells?
Cell Structures and OrganellesLysosomesCell CytotoxicityCellular ProcessesBioconjugation


Posted on April 29, 2022
What enzyme breaks hydrogen bonds in DNA replication?
Cellular ProcessesCell CytotoxicityCell ViabilityPhysiological Probes


Posted on January 28, 2022
Are there any disadvantages for 3D cultures?
Cellular ProcessesCell CytotoxicityCell Cycle AssaysPhysiological Probes


Posted on January 28, 2022
What is a neurosphere formation?
Cell CytotoxicityCellular ProcessesPhysiological ProbesBioconjugation


Posted on January 6, 2022
What is tissue on a chip?
Membrane Potential and ChannelsPhysiological ProbesCell CytotoxicityCellular Processes


Posted on December 20, 2021
What does 7-AAD bind to?
Cell ViabilityCell Cycle AssaysCell CytotoxicityPhysiological Probes


Posted on December 17, 2021
Is 7-AAD toxic to cells?
Cell ViabilityCell Cycle AssaysCellular ProcessesCell Cytotoxicity


Posted on December 10, 2021
Can 7-AAD stain live cells?
Cell ViabilityCell CytotoxicityCellular ProcessesApoptosis and Necrosis


Posted on December 10, 2021
Does 7-AAD stain necrotic cells?
Cell ViabilityCell Cycle AssaysCell CytotoxicityCellular ProcessesFlow Cytometry Reagents


Posted on December 9, 2021
Does pH and staining temperature affect Annexin V-Phosphatidylserine binding?
Cell ViabilityCell CytotoxicityCellular ProcessesApoptosis and NecrosisFlow Cytometry Reagents


Posted on December 9, 2021
Why do I need to use Annexin V Binding Buffer?
Cell ViabilityCell CytotoxicityApoptosis and NecrosisFlow Cytometry Reagents


Posted on October 11, 2021
How are inflammasomes detected?
Cell ViabilityCell CytotoxicityApoptosis and Necrosis


Posted on October 8, 2021
What activates NLRP3 inflammasome?
EnzymesCell MetabolismCell CytotoxicityPolymerases


Posted on September 13, 2021
How does the cell membrane work?
Amplite® Reagents and Assay KitsBioconjugationCell Cytotoxicity


Posted on September 10, 2021
Is BrdU (Bromodeoxyuridine) the only thymidine analog?
Cell Proliferation AssaysBioconjugationCell CytotoxicityCell Viability


Posted on September 10, 2021
Is BrdU (Bromodeoxyuridine) staining performed in vivo?
Cell Proliferation AssaysBioconjugationCell ViabilityCell Cytotoxicity


Posted on September 10, 2021
Can BrdU (Bromodeoxyuridine) be used in vitro?
BioconjugationCell ViabilityCell CytotoxicityApoptosis and Necrosis


Posted on September 10, 2021
How long can BrdU-labeled cells be detected?
Cell ViabilityCell CytotoxicityCellular Processes


Posted on September 10, 2021
How does BrdU (Bromodeoxyuridine) cause mutation?
Cell ViabilityBioconjugationCell Cytotoxicity


Posted on September 9, 2021
Does BrdU (Bromodeoxyuridine) affect the cell cycle?
Cell ViabilityCell CytotoxicityCellular Porcesses


Posted on September 3, 2021
Is BrdU (Bromodeoxyuridine) myelotoxic?
Cell ViabilityCell CytotoxicityCellular Processes


Posted on September 3, 2021
How should BrdU (Bromodeoxyuridine) be disposed of?
Cell CytotoxicityCell ViabilityCellular Processes


Posted on September 3, 2021
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Cell CytotoxicityCell ViabilityPhysiological Probes


Posted on September 3, 2021
Is BrdU (Bromodeoxyuridine) toxic?
Cell ViabilityCell CytotoxicityCellular Processes


Posted on September 3, 2021
What is BrdU (Bromodeoxyuridine) used for?
Cell ViabilityCell CytotoxicityCellular Processes


Posted on September 3, 2021
Is BrdU (Bromodeoxyuridine) light sensitive?
Cell ViabilityCell CytotoxicityCellular Processes


Posted on September 3, 2021
Is BrdU (Bromodeoxyuridine) a stain?
Cell ViabilityCell CytotoxicityCellular Processes


Posted on September 3, 2021
What kind of cells does BrdU (Bromodeoxyuridine) stain?
Cell ViabilityCell CytotoxicityCellular Processes


Posted on September 3, 2021
Is BrdU (Bromodeoxyuridine) a teratogen?
Cell ViabilityCell CytotoxicityCellular Processes


Posted on September 3, 2021
Is BrdU (Bromodeoxyuridine) a biohazard?
Cell ViabilityCell CytotoxicityCellular Processes


Posted on September 3, 2021
Is BrdU (Bromodeoxyuridine) radioactive?
Cell ViabilityCell CytotoxicityCellular Processes


Posted on September 3, 2021
Is BrdU (Bromodeoxyuridine) a carcinogen?
Cell ViabilityCell CytotoxicityCellular Processes


Posted on September 3, 2021
What is BrdU (Bromodeoxyuridine)?
Cell ViabilityCell CytotoxicityCellular Processes


Posted on January 15, 2021
In cells that undergo very rapid cell division, which phases are decreased or eliminated?
Cell ViabilityCell Cytotoxicity


Posted on December 16, 2020
What types of disaggregation techniques are used to make primary cell cultures?
Cell ViabilityCellular ProcessesCell Cytotoxicity


Posted on December 16, 2020
What is the difference between a cell line and a cell strain?
Cell ViabilityCell CytotoxicityCellular Process


Posted on November 25, 2020
How do I determine cell viability?
Cell ViabilityCell CytotoxicityCellular ProcessesEnzymes


Posted on November 24, 2020
What is the difference between cell viability and cell proliferation?
Cell ViabilityCell CytotoxicityCellular Processes


Posted on October 21, 2020
What causes cytotoxicity?
Cell CytotoxicityCell Cycle and ProliferationPhysiological Probes


Posted on October 13, 2020
Is apoptosis good or bad?
Cell CytotoxicityApoptosis and NecrosisCell Viability


Posted on May 9, 2020
Which viability test is better: MTT or trypan blue?
Chemical ReagentsCell Cycle and ProliferationCell CytotoxicityCell Membrane IntegrityCell Proliferation AssaysCell ViabilityCell Viability AssaysLive or Dead


Posted on February 19, 2020
My cells are dying after transfection-what solutions do you recommend?
Cell AssayCell CytotoxicityCell Membrane IntegrityCell ViabilityControlsDNAProteinTransfectioncell culturecell damagecell death


Posted on January 4, 2020
How does phalloidin affect actin assembly?
iFluor dyesActinCell CytotoxicityF-actinPhalloidinactin polymerization inhibitoractin stainingcell-impermeablefluorescently labeled phalloidinphalloidin conjugatesfixed cells


Posted on January 2, 2020
Why does my sample with the most dense cells have the least amount of fluorescence for resazurin?
Cell CytotoxicityCell Assaycell deathcell toxicity


Posted on December 11, 2019
Can I Gram stain living cells?
Cell AssayCell CytotoxicityFixationGram stainingbacteriafluorescence imaginglive cell assay


Posted on October 22, 2019
What are the differences between MTT and WST assays?
AbsorbanceCell ViabilityCell AssayCell CytotoxicityColorimetricWST-8cell proliferation


Posted on June 13, 2019
Do you provide any dyes similar to CFSE that can track cells or monitor cell proliferation?
Cell Cytotoxicity iFluor dyesCytoTellcell proliferationcell-permeable probesflow cytometerfluorescence microplate readerfluorescence microscope


Posted on June 12, 2019
Do you have fixable live cell staining assay kits?
Cell CytotoxicityCell ViabilityMultidrug Resistance (MDR1/P-gp, MRP1)flow cytometryfluorescence imagingfluorescence microplate readerfluorescent microscopefluorescent plate readerlive cell assay


Posted on June 12, 2019
Do you have any cytotoxicity information for your Nuclear LCS dyes?
Cell CytotoxicityPhotostabilityLive or DeadCell ViabilityNuclei Staining


Posted on June 12, 2019
Do you have a ready-to-use cell viability assay kit?
ATPCell Viabilitycell toxicityCell Cytotoxicitychemiluminescenceluminescenseluciferasefirefly luciferaseDTT freeready-to-usehomogenouscell assay